Laboratory for the Certification and Cryopreservation of Animal Germplasm (CGA)

Led by: Marius ZĂHAN
Fields: Molec. Cell. & Sys. Biol.
Institution: University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca (USAMVCN)
Scientists/Total: 7/7
Keywords:  1. Semen, oocyte and pre-implanted embryo quality certification. 2. Sperm, oocyte, embryo and somatic cell cryopreservation. 3. Artificial insemination and embryo transfer in farm animals. 4. In vitro embryo production by IVF (in vitro fertilization) and ICSI (intracytoplasmic sperm injection). 5. Testing the activity of some substances in animal cell culture.
Presentation

1. Developing of germplasm banks in Mangalitsa and Tsigai aboriginal pig and sheep breeds.
2. Researches to optimize the biotechnology of in vitro embryo production.
3. Researches to optimize the sperm and oocyte cryopreservation.
4. Impact study on antioxidant activity of the influence of some substances on cryopreserved and in vitro cultured gametes and embryos.

Infrastructures

- photometer SDM5; cell counter Beckman Coulter Z2 dual; heating plates HT 400 and 200; marine bath Memert and dry bath with Minitube granules; stereomicroscopes with heating platforms Nikon and Olympus SZ 51; microscope Olympus BX41 with heating plate, phase contrast and dark field, computer with soft and monitor TFT 17``; inversed microscope Olympus IX51 with heating plate, phase contrast, fluorescence and monitoring system Colorview II, computer wit soft and monitor TFT 17``
- inversed microscope Olympus IX51, with heating plate, phase contrast, relief contrast, micromanipulation system Narishige and monitoring system Colorview II, computer with soft and monitor TFT 17``;
- incubator with CO2 Memmert INCO2 with external analyzer for CO2;
- device for packing, sealing and inscriptioning of Minitube straws; device for cryopreservation Cryostat C 35 P; deep freezer Sanyo; containers for cryopreservation with liquid nitrogen of 47L, 35L, 20L and 5L
- machine for laboratory glassware washing Mielle; autoclave Raypa; drying oven Memmert UNP400; hoods with sterile laminary flux and II security level Termo and April;
device for air purification; CodaAir Aero700; pure and ultrapure ware making system MilliQ (Elix and Simplicity)
- electronic scale AND GH 202; pH-meter Hanna; micro-osmometer Minitube; spectrophotometer Spectro UVS-2700

Applications

Contact

Cale Mănăştur 3 – 5, 400372
Cluj-Napoca, jud. Cluj, România
Tel: +40 264 596384
Fax: +40 264 593792
eMail: crio...@gmail.com

 

 

 

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